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Fig. 2 | BMC Biochemistry

Fig. 2

From: Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase

Fig. 2

Purification of BfPNK/CPDase. a SDS PAGE from a Ni-NTA purification of BfPNK/CPDase. SF: supernatant flow-through, W: wash, E1-E7: fractions eluted with 500 mM imidazole, M: marker. The calculated size of the protein was 60.3 kDa. b Superdex75 SEC profile of BfPNK/CPDase. The blue curve shows a mixture of monomer and dimer in the absence of any reducing agent, and the green curve shows the monomer in the presence of 5 mM β-ME. The single monomeric peak (green) indicates that the dimer formation is inhibited by β-ME. c SDS-PAGE from a SEC purification of BfPNK/CPDase in the absence of a reducing agent. M: marker, Di: BfPNK/CPDase dimer, Mo: BfPNK/CPDase monomer

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