Fig. 1
Apo-LdtMt2 and adduct structures. a Overlap of the apo- (brown), biapenem- (pink), and tebipenem-complexes (cyan) of LdtMt2. BlgA (aa 56–150) and BlgB (aa 150–250) are Bacterial Immunoglobulin-like (BIg) domains and CD (aa 251–408) is the catalytic domain. b & c Simulating annealing omit map at the active site of LdtMt2-biapenem (b) and LdtMt2-tebipenem (c). The refined structure of the respective catalytic site and adduct are shown. The adduct atoms were omitted during a mock refinement cycle including a torsional simulated annealing step using the refinement program PHENIX. The omit maps were contoured at 3.5 σ level. In both panels the MTOA-adducts are colored green (stereo views in Additional file 1). d Chemical structure of the common β-lactam core of the anticipated non-degraded adducts. e Chemical structure of the observed adduct in both complexes