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Table 1 List of the 16 MAPK genes identified in C. reinhardtii and their sequence characteristics

From: CrMAPK3 regulates the expression of iron-deficiency-responsive genes in Chlamydomonas reinhardtii

Name

Locus Name

ORF (bp)

Amino Acids

kD

pI

Chromosomal localization

Sub cellular location

CrMAPK2

Cre08.g385050

2223

740

79.0

8.61

chr8:4906426..4913322 F

Cytoplasm

CrMAPK3

Cre12.g509000

1062

353

39.8

8.76

chr12:2119590..2122035 R

Cytoplasm

CrMAPK4

Cre17.g745447

2298

765

81.2

7.20

chr17:6855222..6862749 F

Cytoplasm

CrMAPK5

Cre13.g607300

1302

433

48.5

8.88

chr13:5094976..5099387 F

Cytoplasm

CrMAPK6

Cre12.g508900

1128

375

42.5

7.66

chr12:2129460..2133325 R

Nuclear

CrMAPK7

Cre16.g661100

1758

585

63.3

9.7

chr16:2519399..2524020 F

Cytoplasm

CrMAPK8

Cre01.g010000

1170

389

44.0

5.68

chr1:1838122..1842287 F

Cytoplasm

CrMAPK9

Cre12.g538300

1923

640

68.4

9.1

chr12:6473633..6479228 F

Cytoplasm

CrMAPK10

Cre01.g052800

3692

1163

117

9.91

chr1:7342585..7351700 R

Cytoplasm

CrMAPK11

Cre01.g052850

5199

1732

168

9.5

chr1:7356808..7367075 F

Cytoplasm

CrMAPK12

Cre03.g200200

5301

1766

178

9.36

chr3:8184080..8192783 R

Cytoplasm

CrMAPK13

Cre10.g432250

4752

1583

160

8.31

chr10:1948371..1956866 R

Cytoplasm

CrMAPK14

Cre03.g169500

3321

1106

114

9.49

chr3:3709775..3716106 R

Nuclear

CrMAPK15

Cre02.g111014

4212

1403

142

9.15

chr2:5820048..5827789 F

Cytoplasm

CrMAPK16

Cre17.g709500

3237

1078

107

7.93

chr17:1790415..1798683 R

Cytoplasm

CrMAPK17

Cre17.g709750

4956

1651

165

8.56

chr17:1821974..1831590 F

Cytoplasm

  1. F and R represent the forward and reverse directions on the chromosome, respectively. In total, 16 CrMAPK proteins were obtained by BLASTP search using the C. reinhardtii V5.5 proteome database and MAPK proteins from Arabidopsis thaliana as queries. The 16 CrMAPK genes were named based on their name annotated in JGI database. The molecular weights and pIs of the 16 CrMAPK proteins were predicted using ExPASy. The CrMAPK sub-cellular locations were predicted using the Euk-mPLoc2.0 program