Fig. 4
From: CrMAPK3 regulates the expression of iron-deficiency-responsive genes in Chlamydomonas reinhardtii
Analysis of CrMAPK3 RNAi transgenic algal strains. Of the 133 CrMAPK3 RNAi transformants, 99 were colorless or light blue. Among them, the ARS activities of transgenic strains RNAi11, RNAi37, and RNAi62 were significantly decreased under − Fe (a, b). Moreover, the mRNA levels of ARS were significantly decreased (c). The mRNA level of target gene CrMAPK3 was decreased by 97–98% compared with the control (d), indicating that CrMAPK3 in transgenic strains of RNAi11, RNAi37, and RNAi62 has been effectively silenced. The mRNA levels of endogenous CrFOX1 were reduced by 63, 54, and 71%, respectively, at 12, 24, and 48 h post-incubation in –Fe, indicating that CrMAPK3 positively regulates the expression of CrFOX1 gene (e). The data are shown as the means (±SD, n = 3). Significance is indicated as *P < 0.05, **P < 0.01