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Fig. 2 | BMC Biochemistry

Fig. 2

From: CrMAPK3 regulates the expression of iron-deficiency-responsive genes in Chlamydomonas reinhardtii

Fig. 2

An analytical view of the CrMAPK gene family. a An unrooted tree summarizing the evolutionary relationships among the 16 members of the CrMAPK family. Multiple alignments of the 16 CrMAPK protein sequences from C. reinhardtii were conducted using ClustalX 2.1. The phylogenetic tree was constructed using MEGA6. The numbers on each node are Shimodaira-Hasegawa-like test indices of statistical support provided by MEGA6. Bar = 2.0 is a branch length that represents the number of amino acid substitutions per site. The tree shows the 3 phylogenetic subfamilies (numbered I to III and marked with different color backgrounds) with high predictive values. b Intron/exon structures: the gene structures were drawn using the online tool GSDS. As shown in the legend, the exons and introns are indicated by green rectangles and thin lines, respectively. The untranslated regions (UTRs) are indicated by blue boxes. The sizes of exons and introns can be estimated using the scale shown at the bottom. c Schematic representation of the conserved motifs in the 16 CrMAPK proteins elucidated using SMART and PROSITE online. The different domains are indicated by different colored boxes denoted at the bottom right corner. The lengths of the proteins and motifs can be estimated using the scale shown at the bottom

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