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Table 2 The effects of various metal ions and reagents on the activity of BglP

From: Increased enzymatic hydrolysis of sugarcane bagasse by a novel glucose- and xylose-stimulated β-glucosidase from Anoxybacillus flavithermus subsp. yunnanensis E13T

Substances

Relative activity (%)

5 mM

10 mM

Control

100

100

MgCl2

101 ± 1.3

102 ± 1.2

CaCl2

99 ± 0.7

96 ± 0.5

MnCl2

95 ± 1.6

92 ± 1.3

FeCl3

98 ± 1.0

92 ± 0.9

CoCl2

99 ± 0.5

86 ± 1.6

ZnCl2

98 ± 1.0

83 ± 0.4

CuCl2

91 ± 1.4

44 ± 0.5

FeCl2

77 ± 0.8

20 ± 0.6

DTT

99 ± 0.5

98 ± 0.9

Urea

98 ± 1.5

96 ± 1.3

EDTA

96 ± 0.9

82 ± 1.4

β-Mercaptoethanol

99 ± 1.2

92 ± 1.6

DMSOa

5%

10%

97 ± 0.7

90 ± 1.6

Triton X-100a

1%

2%

91 ± 1.0

80 ± 0.5

Tween 80a

82 ± 0.9

73 ± 1.4

SDS

3.4 ± 0.4

ND

  1. The enzymes were pre-incubated for 30 min at 60 °C with each additive before activity measurement with pNPG as substrate. The enzyme activity of BglP without metal ions was taken as 100% (842 ± 15.5 U/mg)
  2. ND not determined. Values are the means ± SD of six experiments (n = 6)
  3. aThe concentrations was percent volume by volume (v/v)