Fig. 1

Effects of temperature and pH on β-glucosidase activity and stability of BglP. a Effect of temperature on enzyme activity. b Effect of temperature on the stability of BglP. The enzyme solution was incubated in Na₂HPO₄-citric acid buffer (pH 7.0, without substrate) at 55 (∆), 60 (■), 65 (□) and 70 °C (○) for different time periods, and the residual activity was measured. c Effect of pH on enzyme activity. The ability was assayed at 60 °C in different buffers: 50 mM Na₂HPO₄-citric acid buffer pH 4.0 to 8.0 and 50 mM Glycine-NaOH buffer pH 8.0 to 10.0. d Effect of pH on stability of BglP. The enzyme was incubated at 25 °C in each buffer pH 6.0 (▲), pH 7.0 (∆), pH 8.0 (■) and pH 9.0 (□) for different time periods, and the residual activity was measured. Values are the means ± SD of six experiments (n = 6)