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Fig. 6 | BMC Biochemistry

Fig. 6

From: Interactions of histatin-3 and histatin-5 with actin

Fig. 6

Kinetics of cross-linking of Fl-histatin-3 and Fl-histatin-5 to G- and F-actin and effect of phalloidin on the extent of cross-linking of Fl-histatin-3, Fl-histatin-5 and Fl-LL-37 to F-actin. a Kinetics of cross-linking of 6 μM Fl-histatin-3 and −5 to 4 μM F-and G-actin. Samples were treated with 0.3 mg/ml TGase at 22 °C for various time intervals, run on SDS-PAGE and evaluated by densitometry. b Effect of 8 μM phalloidin on the extent of cross-linking of 8 μM Fl-histatin-3, Fl-histatin-5 and Fl-LL-37 to 4 μM F-actin. Cross-linking with 0.3 mg/ml TGase for 30 min at 22 °C. Upper panel, quantitative evaluation of SDS-PAGE by densitometry. Lower panel Coomassie blue stained representative SDS-PAGE

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