Fig. 5
Cross-linking of histatins and LL-37 to CaATP-G-actin and MgF-actin by TGase. Samples were treated with TGase at 22 °C for 30 and run on SDS-PAGE and evaluated by densitometry as described in Methods. Lower panels are representative SDS-PAGE-s. Upper panels are quantitative evaluation of SDS-PAGE-s. a Cross-linking 4 μM G- or F-actin with 8 μM histatin-3 or 6 μM LL-37 by 0.4 mg/ml TGase. b Cross linking 4 μM CaATP-G-actin with 8 μM histatin-3 and histatin-5, 4–8 μM Fl-histatin-3, and Fl-histatin-5 by 0.3 mg/ml TGase. Star (*) on 8 in the lower panel marks unlabeled histatin. 8 μM histatin-5-actin cross-linked product could not be evaluated accurately because of its poor separation from the actin band. c Competition between 6 μM Fl-LL-37 and 0–16 μM histatin-3 or histatin-5 for cross-linking 4 μM CaATP-G-actin. Lower panel, SDS-PAGE visualized by fluorescence