Fig. 2

Bundling of F-actin by histatin-3 and histatin-5. Bundling was carried out at low ionic strength. a and b bundling kinetics of 4 μM F-actin was followed by light scattering change as described in Methods. Arrow indicates the time of histatin addition. a Bundling by 2–12 μM histatin-3. b Bundling by 4–40 μM histatin-5. c Extent of bundling of 8 μM F-actin by 0–16 μM histatin-5 and 0–12 μM histatin-3. d Extent of bundling of 8 μM Mg F-actin by 5 μM histatin-3, 12 μM histatin-5 and 5 μM LL-37 at pH 6.5, 7.3 and 8.2. c and d extent of bundling measured by low speed centrifugation and evaluated by densitometry of the SDS-PAGE of supernatants (unbundled actin) as described in Methods. Upper panels quantitative evaluation of bundling, lower panels, representative SDS-PAGE of the supernatants