Fig. 1
Polymerization of 4 μM CaATP-G-actin by histatin-3 and histatin-5. Pyrene-labeled G-actin was polymerized as described in Methods. Kinetics of polymerization was followed by increase in actin fluorescence. Arrow indicates the time of histatin or MgCl2 addition a Polymerization by histatin-3. b Polymerization by histatin-5. c Effect of histatin concentration on the extent of polymerization evaluated by densitometry of the SDS-PAGE of supernatants following high speed centrifugation. d Kinetics of actin polymerization by 8 μM histatin-3, 20 μM histatin-5 and 7 μM LL-37 at pH 6.5, 7.4 and 8.2