Table 4 ΔΔG values for Class A β-lactamases and BLIP Y50 mutants

Nonpolar

Y50A

0.14 ± 0.03

-2.4 ± 0.5

-0.44 ± 0.07

Y50F

2.2 ± 0.4

1.8 ± 0.4

1.4 ± 0.3

Y50L

-0.5 ± 0.1

-0.6 ± 0.2

2.5 ± 0.7

Y50M

-0.7 ± 0.2

-0.9 ± 0.2

1.6 ± 0.5

Y50V

1.1 ± 0.2

1.2 ± 0.3

2.7 ± 0.4

Y50W

1.2 ± 0.3

1.9 ± 0.4

2.1 ± 0.4

Polar

Y50C

2.0 ± 0.4

2.9 ± 0.7

3.0 ± 0.4

Y50N

0.6 ± 0.1

1.7 ± 0.3

2.6 ± 0.7

Y50Q

-0.2 ± 0.1

-0.4 ± 0.1

-0.4 ± 0.1

Y50S

0.7 ± 0.2

0.5 ± 0.1

4.3 ± 0.5

Y50T

0.51 ± 0.09

1.0 ± 0.2

2.9 ± 0.6

Small

Y50G

-0.44 ± 0.06

-0.13 ± 0.03

-0.9 ± 0.2

Y50P

0.7 ± 0.2

2.6 ± 0.6

2.7 ± 0.5

Charged

Y50H

0.042 ± 0.006

1.4 ± 0.3

2.7 ± 0.3

Y50R

1.2 ± 0.2

3.0 ± 0.6

>3.9

Y50K

3.1 ± 0.4

3.6 ± 0.7

2.6 ± 0.7

Y50D

0.46 ± 0.07

3.5 ± 0.9

>4.5

Y50E

0.8 ± 0.1

3.0 ± 0.7

2.9 ± 0.4