| |
BLIP Mutant
|
ΔΔG(kcal/mol) KPC-2
|
ΔΔG(kcal/mol) TEM-1
|
ΔΔG(kcal/mol) Bla1
|
|---|
|
Nonpolar
|
Y50A
|
0.14 ± 0.03
|
-2.4 ± 0.5
|
-0.44 ± 0.07
|
|
Y50F
|
2.2 ± 0.4
|
1.8 ± 0.4
|
1.4 ± 0.3
|
|
Y50L
|
-0.5 ± 0.1
|
-0.6 ± 0.2
|
2.5 ± 0.7
|
|
Y50M
|
-0.7 ± 0.2
|
-0.9 ± 0.2
|
1.6 ± 0.5
|
|
Y50V
|
1.1 ± 0.2
|
1.2 ± 0.3
|
2.7 ± 0.4
|
|
Y50W
|
1.2 ± 0.3
|
1.9 ± 0.4
|
2.1 ± 0.4
|
|
Polar
|
Y50C
|
2.0 ± 0.4
|
2.9 ± 0.7
|
3.0 ± 0.4
|
|
Y50N
|
0.6 ± 0.1
|
1.7 ± 0.3
|
2.6 ± 0.7
|
|
Y50Q
|
-0.2 ± 0.1
|
-0.4 ± 0.1
|
-0.4 ± 0.1
|
|
Y50S
|
0.7 ± 0.2
|
0.5 ± 0.1
|
4.3 ± 0.5
|
|
Y50T
|
0.51 ± 0.09
|
1.0 ± 0.2
|
2.9 ± 0.6
|
|
Small
|
Y50G
|
-0.44 ± 0.06
|
-0.13 ± 0.03
|
-0.9 ± 0.2
|
|
Y50P
|
0.7 ± 0.2
|
2.6 ± 0.6
|
2.7 ± 0.5
|
|
Charged
|
Y50H
|
0.042 ± 0.006
|
1.4 ± 0.3
|
2.7 ± 0.3
|
|
Y50R
|
1.2 ± 0.2
|
3.0 ± 0.6
|
>3.9
|
|
Y50K
|
3.1 ± 0.4
|
3.6 ± 0.7
|
2.6 ± 0.7
|
|
Y50D
|
0.46 ± 0.07
|
3.5 ± 0.9
|
>4.5
|
|
Y50E
|
0.8 ± 0.1
|
3.0 ± 0.7
|
2.9 ± 0.4
|
- BLIP Y50I could not be purified
