| |
BLIP Mutant
|
Ki (nM) KPC-2
|
Ki (nM) TEM-1
|
Ki (nM) Bla1
|
|---|
|
Nonpolar
|
WT
|
1.5 ± 0.2
|
0.5 ± 0.1
|
2.5 ± 0.3
|
|
Y50A
|
1.9 ± 0.3
|
0.010 ± 0.001
|
1.2 ± 0.3
|
|
Y50F
|
15 ± 2
|
10.3 ± 0.8
|
90 ± 14
|
|
Y50L
|
0.7 ± 0.2
|
0.17 ± 0.02
|
160 ± 39
|
|
Y50M
|
0.5 ± 0.1
|
0.11 ± 0.01
|
34 ± 9
|
|
Y50V
|
10 ± 1
|
3.5 ± 0.6
|
200 ± 11
|
|
Y50W
|
1.1 ± 0.2
|
11 ± 1
|
90 ± 11
|
|
Polar
|
Y50C
|
42 ± 6
|
60 ± 10
|
360 ± 5
|
|
Y50N
|
3.8 ± 0.8
|
7.8 ± 0.5
|
180 ± 43
|
|
Y50Q
|
1.0 ± 0.5
|
0.24 ± 0.02
|
1.3 ± 0.3
|
|
Y50S
|
5 ± 1
|
1.1 ± 0.1
|
3110 ± 5
|
|
Y50T
|
3.5 ± 0.4
|
2.5 ± 0.2
|
290 ± 53
|
|
Small
|
Y50G
|
0.72 ± 0.02
|
0.40 ± 0.06
|
0.58 ± 0.07
|
|
Y50P
|
5 ± 1
|
40 ± 3
|
220 ± 28
|
|
Charged
|
Y50H
|
1.4 ± 0.1
|
5.0 ± 0.1
|
230 ± 2
|
|
Y50R
|
11 ± 1
|
69 ± 2
|
>1500
|
|
Y50K
|
260 ± 10
|
195.3 ± 0.2
|
180 ± 47
|
|
Y50D
|
3.2 ± 0.3
|
160 ± 23
|
>4500
|
|
Y50E
|
5.5 ± 0.1
|
76 ± 6
|
331 ± 6
|
- BLIP Y50I could not be purified
