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Fig. 5 | BMC Biochemistry

Fig. 5

From: Copper chelation and interleukin-6 proinflammatory cytokine effects on expression of different proteins involved in iron metabolism in HepG2 cell line

Fig. 5

FTH1 expression levels and intracellular iron concentration. a representative image of FTH1 protein immunoblot relative to total cell extracts, after electrophoresis on 16.5% Tris-Tricine SDS-PAGE. b densitometric analysis of FTH1 protein. The values are normalized to β-actin. c graph relative to intracellular iron concentration in HepG2 cells. Cells were extensively washed, lysed as described in methods, and used for atomic absorption analysis. The iron content was normalized by cellular total protein concentration. All values are expressed as means ± SEM (n = 6). All indicated differences were statistically significant (p < 0.05). *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Expression levels of control condition were normalized to one, and all values are expressed as relative units

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BMC Biochemistry

ISSN: 1471-2091

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