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Fig. 4 | BMC Biochemistry

Fig. 4

From: Copper chelation and interleukin-6 proinflammatory cytokine effects on expression of different proteins involved in iron metabolism in HepG2 cell line

Fig. 4

Western blot analysis of TfR1, and DMT1. HepG2 cells were treated for 16 h in serum-free medium with 300 μM BCS and/or 40 ng/ml IL-6. a representative image of TfR1 protein immunoblot relative to membrane proteins exstracts. Equal amounts of proteins were loaded per lane. b densitometric analysis of TfR1 protein. c representative image of DMT1 protein immunoblot relative to total cell extracts, after electrophoresis on 16.5% Tris-Tricine SDS-PAGE (d) densitometric analysis of DMT1 protein. The values are normalized to β-actin. All values are expressed as means ± SEM (n = 6). All indicated differences were statistically significant (p < 0.05). *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Expression levels of control condition were normalized to one, and all values are expressed as relative units

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