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Fig. 2 | BMC Biochemistry

Fig. 2

From: Copper chelation and interleukin-6 proinflammatory cytokine effects on expression of different proteins involved in iron metabolism in HepG2 cell line

Fig. 2

RT-PCR and Western blot analysis of GPI-Cp expression levels. HepG2 cells were treated for 16 h in serum-free medium with 300 μM BCS and/or 40 ng/ml IL-6. a and b after 16 h of treatment, RNA was isolated, reverse transcribed and subjected to PCR. The amplicons relative to GPI-Cp isoform and β-actin were analysed by agarose gel electrophoresis and intensity of bands was determined by ImageJ 1.47v software (http://imagej.nih.gov/ij). The values of intensity relative to GPI-Cp were normalized by using the β-actin housekeeping gene. c representative image of GPI-Cp isoform protein relative to membrane proteins extracts analysed by western blot. d densitometric analysis of GPI-Cp isoform protein. The values are normalized by β-actin protein level. All values are expressed as means ± SEM (n = 6). All indicated differences were statistically significant (p < 0.05). *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Expression levels of control condition were normalized to one, and all values are expressed as relative units

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