Fig. 5

Tpr Knockdown Specifically Reduces Replication-dependent H1 Variant H1.1 and H1.2 Levels. a Untransfected (UN) U2OS Tet/On cells or U2OS Tet/On cells expressing H1 variants (H1.1, H1.2 and H1x) were treated with either control siRNA or siRNA targeting protein Tpr for 72 h. The cells were simultaneously stained with DAPI (blue), anti-Tpr (Red), and anti-His (Green). Overlay of anti-Tpr and anti-His staining was shown for comparison. b Scatter plot of quantified fluorescence intensities from Fig. 5a. Quantification and regression analysis was performed as described in Experimental Procedures. c Untransfected (UN) U2OS Tet/On cells or U2OS Tet/On cells expressing H1 variants (H1.1, H1.2 or H1x) were treated with either control siRNA or siRNA targeting protein Tpr for 72 h. 20 μg whole cell extracts of each sample were resolved on SDS-PAGE gel, and visualized by immunoblotting with antibodies targeting proteins indicated on the right