Fig. 6
From: Auto-thiophosphorylation activity of Src tyrosine kinase
Thiophosphorylated Src is resistant to dephosphorylation. a Src catalytic domain was preincubated alone or in the presence of MgATP for 30Ā min at 30Ā Ā°C. The samples were analyzed by SDS-PAGE, along with a sample of autophosphorylated Src that had been treated with immobilized GST-PTP1B phosphatase (30Ā min at 30Ā Ā°C). Proteins in the gel were analyzed by Western blotting with anti-Src (pY416) antibody. b Phosphorylated or thiophosphorylated Src catalytic domain was treated with immobilized GST-PTP1B for the indicated amounts of time and analyzed by SDS-PAGE and Western blotting with anti-Src (pY416) antibody. The membrane was stripped of antibody and reprobed with anti-His tag antibody to confirm equal loading of the gel. This experiment was repeated three times with similar results