Fig. 4
From: Auto-thiophosphorylation activity of Src tyrosine kinase
Mass spectrometric analysis of the sites of Src auto-thiophosphorylation. A sample of auto-thiophosphorylated Src was analyzed by SDS-PAGE. The gel band was excised, digested with trypsin, and analyzed by LC-MS/MS as described in Materials and Methods. a MS/MS scan of the peptide containing the major autophosphorylation site (Tyr416), LIEDNEYTAR. b MS/MS scan of the peptide containing the secondary thiophosphorylation site, WTAPEAALYGR