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Fig. 3 | BMC Biochemistry

Fig. 3

From: Auto-thiophosphorylation activity of Src tyrosine kinase

Fig. 3

Measurements of the extent of Src auto-thiophosphorylation. a Src kinase domain was incubated in the presence of NiATPĪ³S or MgATP, and the time course of reaction was monitored by nondenaturing gel electrophoresis. Detection was with Coomassie blue staining. b The stoichiometry of Src auto-thiophosphorylation was determined by carrying out reactions in the presence of [35S]-labeled ATPĪ³S. Error bars show standard deviations. The experiments in panels a and b were carried out at least three times with similar results

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