Skip to main content

Table 4 The half-life of wild-type and mutants LacA at various initial oNPG concentrations (w/v) and temperatures

From: Effect of mutations to amino acid A301 and F361 in thermostability and catalytic activity of the β-galactosidase from Bacillus subtilis VTCC-DVN-12-01

Enzyme

Half-life (h) at various initial oNPG concentrations (w/v) and temperatures

 

45 °C

50 °C

0 %

5 %

10 %

15 %

0 %

5 %

10 %

15 %

WT

3.13 ± 0.14

29.36 ± 0.11

16.11 ± 0.15

11.81 ± 0.09

0.42 ± 0.03

3.75 ± 0.04

2.98 ± 0.06

1.79 ± 0.03

A301V

7.6 ± 0.19

6.52 ± 0.09

7.15 ± 0.12

6.79 ± 0.21

0.57 ± 0.03

0.81 ± 0.03

0.73 ± 0.008

0.49 ± 0.01

F361Y

83.89 ± 0.78

99 ± 0.33

90 ± 0.24

92.4 ± 0.52

5.4 ± 0.15

6.16 ± 0.23

7.1 ± 0.14

6.21 ± 0.08

  1. The activity of β-galactosidase was determined in 100 mM buffer Z (pH 7.0) at 55 °C with substrate oNPG (4 mg/ml)