Enzyme | Half-life (h) at various initial oNPG concentrations (w/v) and temperatures | |||||||
---|---|---|---|---|---|---|---|---|
45 °C | 50 °C | |||||||
0 % | 5 % | 10 % | 15 % | 0 % | 5 % | 10 % | 15 % | |
WT | 3.13 ± 0.14 | 29.36 ± 0.11 | 16.11 ± 0.15 | 11.81 ± 0.09 | 0.42 ± 0.03 | 3.75 ± 0.04 | 2.98 ± 0.06 | 1.79 ± 0.03 |
A301V | 7.6 ± 0.19 | 6.52 ± 0.09 | 7.15 ± 0.12 | 6.79 ± 0.21 | 0.57 ± 0.03 | 0.81 ± 0.03 | 0.73 ± 0.008 | 0.49 ± 0.01 |
F361Y | 83.89 ± 0.78 | 99 ± 0.33 | 90 ± 0.24 | 92.4 ± 0.52 | 5.4 ± 0.15 | 6.16 ± 0.23 | 7.1 ± 0.14 | 6.21 ± 0.08 |