Fig. 3From: Effect of mutations to amino acid A301 and F361 in thermostability and catalytic activity of the β-galactosidase from Bacillus subtilis VTCC-DVN-12-01pH stability of Lac-WT, Lac-301 V and LacA-361Y. The purified LacA-WT, Lac-301 V and LacA-361Y were incubated in 100 mM Na-phosphate buffer pH 4. The relative activity of β-galactosidase toward oNPG (4 mg/ml) was determined in 100 mM buffer Z (pH 7.0) at 55 °CBack to article page