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Fig. 2 | BMC Biochemistry

Fig. 2

From: Effect of mutations to amino acid A301 and F361 in thermostability and catalytic activity of the β-galactosidase from Bacillus subtilis VTCC-DVN-12-01

Fig. 2

Temperature (a) and pH optimum (b) of Lac-WT, Lac-301 V and LacA-361Y. β-Galactosidase activity toward oNPG was determined in 100 mM buffer Z (pH 7.0). The pH was determined in different buffers by varying pH values from 4.0 to 9.0 at 55 °C using substrate oNPG (4 mg/ml). Na-acetate buffer pH 4–6, Na-phosphate buffer pH 6.0–8.0, Tris–HCl buffer pH 8.0–9.0

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