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Fig. 1 | BMC Biochemistry

Fig. 1

From: Effect of mutations to amino acid A301 and F361 in thermostability and catalytic activity of the β-galactosidase from Bacillus subtilis VTCC-DVN-12-01

Fig. 1

SDS-PAGE analysis of the level expression (A) and puried mutant proteins (B) of wild-type and mutants LacA. a. LacA-WT and mutants were expressed to homogeneity. Then total protein of cells were checked on SDS-PAGE to analysis the expression of LacA-WT and mutants. b. LacA-WT and mutants were puried to homogeneity using nickel chelate affinity chromatography. Homogeneous fractions were collected to analysis on SDS-PAGE. The protein samples were run on a 12.5 % SDS reducing gel and stained with Coomassie Briliant Blue R250. Lane 1–7: WT, E62V, R77W, A191V, A301V, F361Y and A524T, respectively; lane M: molecular marker

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BMC Biochemistry

ISSN: 1471-2091

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