Fig. 5
Infection of TSC1f/f LysM-Cre+ mice with M. tuberculosis resulted in lower mycobacterial counts at the time of the arrival of adaptive immune cells. Both TSC1f/f LysM-Cre+ and LysM-Cre- mice were aerogenously infected with M. tuberculosis H37Rv with 200-300 bacilli per lung. Bacterial burdens were measured by CFUs in the lungs (a) at 1, 2, 3, and 5 weeks post-aerosol infection and in the spleens (b) at 3 and 5 weeks post-aerosol infection (n = 4 per time point). c Cytokine secretion from lung homogenates was determined by Luminex at 3 weeks post-aerosol infection. The data were shown as mean ± SD is shown (n = 4). d Flow cytometry analysis of MHCII expression in TSC1 WT and KO macrophages at 3 weeks after M. tuberculosis infection is shown. MFI ± SD is shown (n = 4). MFI = mean fluorescence intensity. *, p < 0.05