Fig. 7

Effect of amino acid modifying reagents on nuclease activity of hPLSCR1. a Kunitz assay: Kunitz assay was used to calculate specific activity of hPLSCR1 (20 pmol) treated with 4 mM of each of the amino acid modifiers including NEM, PG, DEPC, AEBSF along with native hPLSCR1 (untreated). Graph shows the percentage activity of modifier treated hPLSCR1 compared to the untreated hPLSCR1. b DEPC dose dependence studies on nuclease activity: Kunitz assays were used to calculate specific activity of hPLSCR1 treated with an increasing concentration of DEPC (2 mM, 4 mM, 8 mM, and 16 mM) and plotted. c Effect of protein modifying reagents on nuclease activity (gel assay): Nuclease assays for hPLSCR1 treated with 4 mM of NEM, AEBSF, DEPC, and PG at 37 °C for 60 min were visualized in 1 % agarose gel. * denotes statistical significance at p < 0.05, ** denotes statistical significance at p < 0.005; ns – not significant. Experiments were performed in triplicates and error bars denote standard deviation