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Fig. 2 | BMC Biochemistry

Fig. 2

From: Identification and characterization of the novel nuclease activity of human phospholipid scramblase 1

Fig. 2

Topo IIα decatenation assay in presence of hPLSCR1. Decatenation assay was performed for topoisomerase IIα in the presence of hPLSCR1 as described in 'Methods'. 200 ng of kinetoplast DNA (kDNA) is treated with 1 U of topo IIα for 15 min at 37 °C and was then visualized on a 1 % agarose gel. Control denotes negative control where only kDNA is present. Lane 1 and lane 2 corresponds to the decatenation assay performed with topo IIα pre-treated with 2 pmol of hPLSCR1 and 20 pmol of hPLSCR1 respectively. Lane 3 corresponds to the decatenation assay in the absence of topo IIα where only hPLSCR1 was added to kDNA

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BMC Biochemistry

ISSN: 1471-2091

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