From: Tubulin is a molecular target of the Wnt-activating chemical probe
Cellular signal | Cell lines | Cell density (cells/well) | Ligand | Incubation time with compounds | Original materials or references |
---|---|---|---|---|---|
cAMP response element (CRE) signal | HEK293T | 5,000 | Forskolin (1 μM) | 5 h | pGL4.29 (Promega) |
Nuclear factor of activated T-cells (NFAT) signal | HEK293T | 5,000 | Ionomycin (1 μM) PMA (10 ng/mL) | 5 h | pGL4.30 (Promega) |
Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signal | HEK293T | 10,000 | TNFα (3 ng/mL) | 20 h | pGL4.32 (Promega) |
Serum response element (SRE) signal | HEK293T | 20,000 | FBS (15Â %) PMA (30Â ng/mL) | 20Â h | pGL4.33 (Promega) |
Serum response factor (SRF) signal | HEK293T | 20,000 | FBS (15Â %) | 5Â h | pGL4.34 (Promega) |
p53 signal | HEK293T | 10,000 | Doxorubicin (1 μM) | 20 h | [43] |
E2F signal | HEK293T | 20,000 | FBS (15Â %) | 20Â h | [44] |
Activating transcription factor 6 signal | HEK293T | 5,000 | Thapsigargin (30 nM) | 5Â h | [45] |
Hedgehog signal | NIH3T3 | 7,500 | mouse sonic hedgehog | 20Â h | [46] |
Hypoxia-inducible factor 1 (HIF1) signal | HEK293T | 10,000 | hypoxia | 20Â h | [47] |
Nuclear factor erythroid 2-related factor 2 (Nrf2) signal | HEK293T | 5,000 | tert-butylhydroquinone (20 μM) | 20 h | pGL4.37 (Promega) |
SMAD signal | HEK293T | 10,000 | TGFβ (0.2 ng/mL) | 20 h | [48] |
Wnt signal | HEK293T | 10,000 | Wnt3a | 20Â h | [49] |
Wnt signal | SW480 | 10,000 | no ligand (constitutive active) | 20Â h | [49] |
IL17 signal | Jurkat | 5,000 | Ionomycin (400 nM) PMA (4Â ng/mL) | 5Â h | [50] |