Fig. 5
From: Tubulin is a molecular target of the Wnt-activating chemical probe
Effect of AMBMP on the cellular tubulin network and mitotic spindles. (A) The cellular tubulin network (green) was observed by fluorescence microscopy. Hoechst 33342 was used as a nuclear marker (blue). (a) control. (b) 3 μM SB216763. (c) 3 μM AMBMP. (d) 3 μM Nocodazole. Scale bar, 10 μm. (B) Control and compounds-treated MDA-MB-231 cells were stained with α-tubulin (green), phospho-histone H3 (red), and nuclei (blue). Phosphorylation at a highly conserved serine residue (Ser10) in the histone H3 is a key marker during the mitotic phase of the cell cycle. (a) control. (b) 30 nM nocodazole. (c) 0.3 μM nocodazole. (d) 3 μM nocodazole. (e) 30 nM AMBMP. (f) 0.3 μM AMBMP. (g) 3 μM AMBMP. Scale bar, 10 μm