Fig. 4
From: Tubulin is a molecular target of the Wnt-activating chemical probe
Target identification of AMBMP and its binding activity to tubulin. a The Tanimoto structural similarities and Pearson’s correlation coefficients (activity versus activity) were calculated against AMBMP. b SB216763, AMBMP, and nocodazole were evaluated in a tubulin polymerization assay. The results are the mean of 3 replicate experiments (with SD not shown for graphical simplicity). c AMBMP induced intrinsic tryptophan fluorescence spectra changes of tubulin. The results are the mean of 3 replicate experiments (with SD not shown for graphical simplicity)