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Fig. 3 | BMC Biochemistry

Fig. 3

From: Tubulin is a molecular target of the Wnt-activating chemical probe

Fig. 3

A cell-based assay for a screening campaign of cellular senescence morphology inducers by fluorescence microscopy. a MDA-MB231 cells were treated with 1 mM sodium butyrate. Hoechst 33342 was used as a nuclear marker (blue) and CMFDA was used to mark cytosols (green). Scale bar, 10 μm. b A compound selection scheme for the discovery of senescence inducers. AMBMP was obtained as a hit compound through the screening campaign. c Hit compound results from the screening campaign. Fold changes in the cellular area at 3 μM concentrations were calculated for the compounds with a custom-made image analysis algorithm. d Activity of the Wnt reporter gene assay with a potent GSK3β inhibitor, SB216763, and AMBMP is shown. The results are the mean of 3 replicate experiments (mean ± SD). e MDA-MB231 cells were treated with 3 μM SB216763, 3 μM AMBMP, and 1 mM sodium butyrate. Hoechst 33342 was used as a nuclear marker (blue) and CMFDA was used to mark cytosols (green). Scale bar, 10 μm

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