Fig. 2From: Molecular characterization of protein kinase C delta (PKCδ)-Smac interactionsPKCδ and Smac bind directly to each other. a–c His-Smac was incubated with GST-PKCδ or GST for 1 h before GST-immunoprecipitation. In b, His-Smac was incubated with GST-PKCδ for all samples. Fractions were collected and analyzed with Western blot. 20 nM TPA and/or 2 μM GF109203X (GFX) were included in the binding reactions where indicated. Figures are representatives from three experiments. Asterisk indicates non-specific band. c Quantification of signal intensity of Smac bands in the IP-fractions from (b). Band intensities were normalized to input fractions and control. Data represents mean ± SEM from three independent experiments. * indicates p < 0.05Back to article page