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Fig. 1 | BMC Biochemistry

Fig. 1

From: Molecular characterization of protein kinase C delta (PKCδ)-Smac interactions

Fig. 1

PKC activation enhances the interaction between PKCδ and Smac in cells. a Schematic of Smac structure. b, c COS-7 cells were transfected with vectors encoding EGFP-tagged full-length PKCδ and Smac-FLAG. After transfection, indicated cells were treated with 16 nM TPA (b) with or without 2 μM GF109203X (c) for 16 h. Lysates were then used for GFP-immunoprecipitation. d MCF-7 cells were transfected with a vector encoding Smac-HSV. After transfection, indicated cells were treated with 16 nM TPA for 16 h. Lysates were then used for PKCδ-immunoprecipitation. Figures are representatives from three experiments. Asterisk indicates non-specific band

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