Fig. 1
From: Conserved motif VIII of murine DNA methyltransferase Dnmt3a is essential for methylation activity
The amino acid residues subjected to mutational analysis. a Multiple sequence alignment of mammalian Dnmt3a (from mouse and human), Dnmt3b and Dnmt1 with prokaryotic M.SssI, M.HpaI and M.HhaI. Sequences were aligned by ClustalW algorithm and shaded using BoxShade server. b Model of Dnmt3a-CD (green) with short DNA molecule (blue) adopted by superposition of the M.HhaI-DNA complex structure [20] onto Dnmt3a-CD/Dnmt3L tetramer structure [16]. Top (top panel) and side (bottom panel) views. The motifs IV (PCN), VI (ENV) and VIII (QxRxR) are shown