Fig. 2From: Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa Acetate release assay: The indicated acetylpolyamines were added to A) PA0321 and B) PA1409 after the equilibrium of the assay mix was formed (ca. 22 min) the activities were calculated from the linear increase in NADH concentration after adding the acetylated polyamines. Protein concentrations were 200 nM for PA0321 and 100nM for PA1409Back to article page