Fig. 6From: Linalool isomerase, a membrane-anchored enzyme in the anaerobic monoterpene degradation in Thauera linaloolentis 47LolFormation of geraniol by linalool isomerase. The most enriched protein sample after sucrose gradient centrifugation and precipitation was used: 200 μL sample (1.04 mg mL−1 protein), 5 mM dithionite, 1 μL (R,S)-linalool (≈30 mM). Incubation was performed at 28 °C and individual samples were extracted with 200 μL n-hexane after 0, 2 and 4 h (from bottom to top) and measured by gas chromatography (Shimadzu GC 14A, see methods). Retention time of geraniol was at 12.55 minBack to article page