Skip to main content

Table 3 Effect of different additives on Lip3 activity

From: Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries

  Relative activity %
Additives Concentration Concentration
1 mM 10 mM
Control 100 ± 0.007 100 ± 0.004
β-mercaptoethanol 96.0 ± 0.005 56.0 ± 0.002
EDTA 91.0 ± 0.001 78.7 ± 0.003
DTT 106.0 ± 0.021 59.0 ± 0.005
PMSF 77.0 ± 0.011 55.0 ± 0.004
  1. Esterase activities were measured toward various compounds at 35 °C in presence of 0.1 M Tris-HCl pH 8.0