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Table 3 Effect of different additives on Lip3 activity

From: Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries

 

Relative activity %

Additives

Concentration

Concentration

1 mM

10 mM

Control

100 ± 0.007

100 ± 0.004

β-mercaptoethanol

96.0 ± 0.005

56.0 ± 0.002

EDTA

91.0 ± 0.001

78.7 ± 0.003

DTT

106.0 ± 0.021

59.0 ± 0.005

PMSF

77.0 ± 0.011

55.0 ± 0.004

  1. Esterase activities were measured toward various compounds at 35 °C in presence of 0.1 M Tris-HCl pH 8.0
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BMC Biochemistry

ISSN: 1471-2091

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