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Table 2 Kinetic parameters for Lip3

From: Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries

Substrate k cat (s−1) K M (M∙10−3) s = k cat / K M (sec−1∙M−1∙103)
pNP-acetate 1198 ± 200 2.38 ± 0.56 503.3 ± 214.5
pNP-butanoate 218.0 ± 15.5 1.05 ± 0.16 206.5 ± 48.6
pNP-pentanoate 152.8 ± 9.82 1.52 ± 0.20 100.2 ± 19.7
pNP-octanoate 100.0 ± 3.8 0.36 ± 0.04 271.0 ± 44.0
pNP-decanoate 29.9 ± 1.5 1.03 ± 0.12 29.3 ± 10.1
  1. Esterase activity was measured at 35 °C for five minutes in presence of 0.1 M Tris-HCl pH 8.0