Skip to main content

Table 2 Kinetic parameters for Lip3

From: Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries

Substrate

k cat (s−1)

K M (M∙10−3)

s = k cat / K M (sec−1∙M−1∙103)

pNP-acetate

1198 ± 200

2.38 ± 0.56

503.3 ± 214.5

pNP-butanoate

218.0 ± 15.5

1.05 ± 0.16

206.5 ± 48.6

pNP-pentanoate

152.8 ± 9.82

1.52 ± 0.20

100.2 ± 19.7

pNP-octanoate

100.0 ± 3.8

0.36 ± 0.04

271.0 ± 44.0

pNP-decanoate

29.9 ± 1.5

1.03 ± 0.12

29.3 ± 10.1

  1. Esterase activity was measured at 35 °C for five minutes in presence of 0.1 M Tris-HCl pH 8.0