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Table 1 Comparison of the mlDL fermentative activities and half-maximal diapasones

From: A comparison of the enzymatic properties of three recombinant isoforms of thrombolytic and antibacterial protein—Destabilase-Lysozyme from medicinal leech

 

Muramidase activity (in lysozyme units)

Lytic activity

Isopeptidase activity

Half-maximal pH and I values for

in activity units

Km, μM

kkat, ×10−2 s−1

muramidase activity

lytic activity

isopeptidase activity

mlDL-Ds1

5200 ± 450

0. 079 ± 0.005

850 ± 70

40

7

pH 2.5–3, I = 0–0.25 M;

pH 4.5–10

pH 4.5–7.5

mlDL-Ds2

2450 ± 250

0. 035 ± 0.003

660 ± 60

50

8

pH 4.5–12, I = 0–0.05 M

I = 0–0.4 M

I = 0–0.1 M

mlDL-Ds3

10,000 ± 600

0. 136 ± 0.005

1780 ± 160

20

5