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Table 3 Surface plasmon resonance binding analysis of BlMan5_8 to oligosaccharides

From: The GH5 1,4-β-mannanase from Bifidobacterium animalis subsp. lactis Bl-04 possesses a low-affinity mannan-binding module and highlights the diversity of mannanolytic enzymes

Enzyme

Substrate

K d (mM)

n a

BlMan5_8

M4

5.1 ± 0.8

0.78

 

M5

3.7 ± 0.3

0.94

 

M6

0.58 ± 0.05

0.92

 

Cellotetraose

n.d.b

BlMan5_8-ΔCBM10

M4

n.d.b

 

M5

1.5 ± 0.3

0.36

 

M6

0.24 ± 0.42

0.53

 

Cellotetraose

n.d.b

  1. a Estimated stoichiometry of binding to the immobilized protein
  2. b Not detected