From: The GH5 1,4-β-mannanase from Bifidobacterium animalis subsp. lactis Bl-04 possesses a low-affinity mannan-binding module and highlights the diversity of mannanolytic enzymes
Enzyme
Substrate
K d (mM)
n a
BlMan5_8
M4
5.1 ± 0.8
0.78
M5
3.7 ± 0.3
0.94
M6
0.58 ± 0.05
0.92
Cellotetraose
n.d.b
–
BlMan5_8-ΔCBM10
1.5 ± 0.3
0.36
0.24 ± 0.42
0.53