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Table 2 Kinetic parameters for BlMan5_8 on LBG-lv and M5

From: The GH5 1,4-β-mannanase from Bifidobacterium animalis subsp. lactis Bl-04 possesses a low-affinity mannan-binding module and highlights the diversity of mannanolytic enzymes

Substrate

Enzyme

k cat

K m

k cat/K m

LBG-lv

BlMan5_8

1828 ± 87 s−1

1.58 ± 0.23 g · L−1

1157 ± 177 L · s−1 · g−1

 

BlMan5_8-ΔCBM10

2005 ± 179 s−1

1.75 ± 0.47 g · L−1

1146 ± 324 L · s−1 · g−1

M5

BlMan5_8

97 ± 2 s−1

1.09 ± 0.11 mM

89 ± 9 s−1 mM−1

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BMC Biochemistry

ISSN: 1471-2091

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