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Fig. 6 | BMC Biochemistry

Fig. 6

From: The GH5 1,4-β-mannanase from Bifidobacterium animalis subsp. lactis Bl-04 possesses a low-affinity mannan-binding module and highlights the diversity of mannanolytic enzymes

Fig. 6

Phylogenetic tree and sequence alignment of CBM10 sequences. Phylogenetic tree constructed based on sequence alignment of available CBM10 sequences (a) and alignment of the CBM10 of BlMan5_8 and putative CBM10 sequences of characterized enzymes as predicted by dbCAN (b). The sub-tree containing BlMan5_8 is highlighted in black. The characterized enzymes BlMan5_8, SlMan5A [54], CjMan5A [22] and CjXyn10A [55] are indicated. The dbCAN tool (http://csbl.bmb.uga.edu/dbCAN/index.php) was used to obtain putative CBM10 sequences and the sequence of the SlMan5A CBM10 was added manually. Multiple sequence alignment of CBM10 from the five characterized enzymes is shown. The proposed binding residues Tyr8, Thr10, Trp22, Trp24 and Gln39 in the CjXyn10A CBM10 structure [52] are indicated by arrows. The unique insertion in the BlMan5_8 clade preceding the binding residue Trp22 is indicated with a horizontal parenthesis

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BMC Biochemistry

ISSN: 1471-2091

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