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Fig. 5 | BMC Biochemistry

Fig. 5

From: The eukaryotic translation initiation factor 3f (eIF3f) interacts physically with the alpha 1B-adrenergic receptor and stimulates adrenoceptor activity

Fig. 5

The proline-alanine rich amino terminal of eIF3f is not essential for adrenoceptor activation. The experimental conditions were the same as in Fig. 3. eIF3fΔ91 is a genetic construct where the first 91 amino acids of eIF3f were removed (proline- and alanine-rich N-terminal region). a Standard Gαq/11 activity reaction without catecholamine stimulation. b Standard Gαq/11 activity reaction with catecholamine stimulation. c Gαq/11 activity reaction without catecholamine stimulation and added eIF3fΔ91 protein. d Gαq/11 activity reaction with catecholamine stimulation and added eIF3fΔ91 protein. Data and error bars represent mean ± S.E. for three independent experiments; ** p < 0.005 with respect to the control (a), and ɫ p < 0.0005 between C and D

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