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Fig. 3 | BMC Biochemistry

Fig. 3

From: The eukaryotic translation initiation factor 3f (eIF3f) interacts physically with the alpha 1B-adrenergic receptor and stimulates adrenoceptor activity

Fig. 3

eIF3f promotes adrenoceptor activity upon catecholamine stimulation. Adrenoceptor activity is measured on the basis of in vitro agonist-promoted binding of [gamma-32P]GTP to G protein alpha subunits [16], in the presence of cell membrane fractions, and isolated subsequently by immunoprecipitation. In each condition, Gαq/11 immunoprecipitates were analyzed by scintillation for radiolabeled GTP binding. a Standard Gαq/11 activity reaction without catecholamine stimulation. b Standard Gαq/11 activity reaction with catecholamine stimulation. c Gαq/11 activity reaction without catecholamine stimulation and added eIF3f protein. d Gαq/11 activity reaction with catecholamine stimulation and added eIF3f protein. e Gαq/11 activity reaction with catecholamine stimulation and added eIF3f protein, using membrane fractions obtained from alpha 1B-ADR over-expressed A549 cultures. f Standard Gαq/11 activity reaction with catecholamine stimulation, using membrane fractions obtained from over-expressed alpha 1B-ADR A549 cultures. Data and error bars represent means ± standard error (S.E.) for three independent experiments; *p < 0.05 and **p < 0.005 with respect to the controls (A and F), ɫ p < 0.005 between C and D, and Ϯ p < 0.005 between D and E

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