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Fig. 1 | BMC Biochemistry

Fig. 1

From: The eukaryotic translation initiation factor 3f (eIF3f) interacts physically with the alpha 1B-adrenergic receptor and stimulates adrenoceptor activity

Fig. 1

eIF3f is immunodetected in a protein complex of approximately 120 kDa in native conditions. Cells under exponential growth were lysed in a native-permissive buffer and 50 μg of total protein were subjected to electrophoresis in native 8 % polyacrilamide gels. Western blots were performed with an anti-eIF3f antibody (MW: protein molecular weight standard). Immunodetected protein complex in human A549 cells, a human lung carcinoma epithelial cell line; HepG2, a human hepatocellular carcinoma cell line; Ramos, a human Burkitt’s lymphoma cell line; and in murine MC3T3-E1 pre-osteoblast cells. A single prominent band of approximately 120 kDa is evident in all cell types

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BMC Biochemistry

ISSN: 1471-2091

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