Fig. 3

Effect of the laforin/malin complex on the enzymatic activity of the PKM isoforms. a Crude extracts from HEK293 cells expressing the indicated plasmids were used to determine the pyruvate kinase enzymatic activity as indicated in Methods. The results were plotted as Units/mg total protein in the extracts (data are the mean of at least three independent experiments; bars indicate standard deviation; **p < 0.01). b Crude extracts from panel a were also analyzed by SDS-PAGE and immunoblotting using the indicated antibodies. c HEK293 cells expressing myc-PKM1 were transfected with the indicated plasmid. Crude extracts were obtained and the pyruvate kinase enzymatic activity measured as in panel a) (data are the mean of at least three independent experiments; bars indicate standard deviation; **p < 0.01). Crude extracts were also analyzed by SDS-PAGE and immunoblotting using the indicated antibodies (right panel)