Evaluating the role of a trypsin inhibitor from soap nut (Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization

Table 2 Summary of purification of soap nut seed protease inhibitor

Preparation	Volume (ml)	Total protein (mg)	Total Trypsin inhibitory activity (Units) TIU0 × 10³	Specific activity TIA × 10² (Units/mg) protein	Yield %	Fold purification
Crude extract	250	4030	63.10	0.156	100	1.00
Acetone fractionated extract	225	2644	54.81	0.207	86.60	1.33
Heat treated extract	200	2334	53.10	0.227	84.15	1.44
50 % Ammonium sulphate	30	172.5	23.78	1.370	37.69	8.78′
CM – Cellulose	27	112	14.74	1.440	23.36	9.23
Sephadex G-100	12	52	13.2	2.54	20.92	16.28

Yield and fold purification were calculated on the basis of TIU and TIA respectively
TIU trypsin inhibitory Units, TIA trypsin inhibitory activity

ISSN: 1471-2091