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Fig. 4 | BMC Biochemistry

Fig. 4

From: Evaluating the role of a trypsin inhibitor from soap nut (Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization

Fig. 4

a SDS – PAGE. Direction of migration is from top (cathode) to bottom (anode). (1) Molecular weight markers: Phosphorylase b (97.4 kDa), Bovine serum albumin (66 kDa), Ovalbumin (43 kDa), Carbonic anhydrase (29 kDa), Lysozyme (14.3 kDa). (2). Purified SNTI (coomassie brilliant blue stained). (3) Purified SNTI (silver stained). *(1) to (3) were kept at 100 °C for 3 min with SDS and 2 mercaptoethanol. b Molecular weight determination of SNTI by SDS PAGE on 12 % slab gel. Plot of distance migrated against log molecular weight of standard proteins. BSA −66 K.Da Ovalbumin −43 K.Da Carbonicanhydrase −29 K. DaLysozyme −14.3 K.Da

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BMC Biochemistry

ISSN: 1471-2091

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