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Fig. 1 | BMC Biochemistry

Fig. 1

From: Evaluating the role of a trypsin inhibitor from soap nut (Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization

Fig. 1

Ion exchange chromatography of SNTI on CM-Cellulose. One hundred seventy two milligram of the ammonium sulphate fractionated sample (0–50 %) was applied on to the column (2 × 80 cm) in 0.1 M sodium phosphate buffer (pH 5.8) and the adsorbed proteins were eluted with stepwise gradient in the buffer. Fractions of 5 ml were collected at a flow rate of 60 ml per hour. The protein was monitored by absorbance at 280 nm. * When the elution was done with a gradient of 0.1 to 1.0 M NaCl a single but broad peak was obtained (Results not shown. To obtain a sharp peak, the elution was performed using stepwise gradient

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