Fig. 8

Complex II–III coupled enzymatic activity is increased in brain compared with liver mitochondria. a Complex II–III coupled enzymatic activity was assessed by following the reduction of ferricytochrome c to ferrocytochrome c at 550 nM by 40 μg of isolated mitochondria. A baseline was recorded after the addition of ferricytochrome c (15 μM), alamethacin (30 μg/mL), and rotenone (2 μM). The reaction was initiated by the addition of freshly isolated mitochondria (200 μg/mL) preincubated with succinate (20 mM) at 30 °C for 20 min in potassium phosphate buffer. Malonate (20 mM) was added in parallel wells, and the specific activity of complex II was obtained by subtracting the malonate insensitive activity. For brain mitochondria n = 9 and for liver mitochondria n = 8. b The expression level of Slc25a10 was determined in brain and liver tissue, respectively, by RT-PCR and normalized to Gapdh. Three independent experiments were performed in triplicate